c314s plasmids Search Results


c314s plasmids  (Thermo Fisher)
90
Thermo Fisher c314s plasmids
Cys-314 is important for ASBT S-acylation and transport function. A and B, cellular expression (A) and [3H]TC uptake (B) in HEK293 cells transfected with WT or <t>C314S</t> ASBT-V5. n = 3; ****, p < 0.0001 compared with WT. C, Western blotting of bound (S-acylated) and unbound (nonacylated) acyl-RAC fractions from HEK293 cells transfected with WT or C314S ASBT-V5 (representative of three Western blots with similar results). D, a representative Western blotting for cell surface biotinylation of WT and C314S ASBT-V5 fusion proteins.
C314s Plasmids, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c314s plasmids/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
c314s plasmids - by Bioz Stars, 2026-03
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Cys-314 is important for ASBT S-acylation and transport function. A and B, cellular expression (A) and [3H]TC uptake (B) in HEK293 cells transfected with WT or C314S ASBT-V5. n = 3; ****, p < 0.0001 compared with WT. C, Western blotting of bound (S-acylated) and unbound (nonacylated) acyl-RAC fractions from HEK293 cells transfected with WT or C314S ASBT-V5 (representative of three Western blots with similar results). D, a representative Western blotting for cell surface biotinylation of WT and C314S ASBT-V5 fusion proteins.

Journal: The Journal of Biological Chemistry

Article Title: S -acylation modulates the function of the apical sodium-dependent bile acid transporter in human cells

doi: 10.1074/jbc.RA119.011032

Figure Lengend Snippet: Cys-314 is important for ASBT S-acylation and transport function. A and B, cellular expression (A) and [3H]TC uptake (B) in HEK293 cells transfected with WT or C314S ASBT-V5. n = 3; ****, p < 0.0001 compared with WT. C, Western blotting of bound (S-acylated) and unbound (nonacylated) acyl-RAC fractions from HEK293 cells transfected with WT or C314S ASBT-V5 (representative of three Western blots with similar results). D, a representative Western blotting for cell surface biotinylation of WT and C314S ASBT-V5 fusion proteins.

Article Snippet: Cells were transfected with 1 μg/well or 12 μg/dish of ASBT-V5 WT or C314S plasmids using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions.

Techniques: Expressing, Transfection, Western Blot